Genome assembly is a method for re-constructing a genome from a large number of (short- or long-) DNA fragments (reads) when no reference genome is available.
During this lesson you will use sequencing reads from different technologies:
1) short reads from Illumina
2) and long read from Oxford Nanopore
Prerequisites
This lesson assumes a working understanding of the bash shell. If you haven’t already completed the Shell Genomics lesson, and aren’t familiar with the bash shell, please review those materials before starting this lesson.
This lesson also assumes some familiarity with biological concepts, including the structure of DNA, nucleotide abbreviations, and the concept of genomic variation within a population.
This lesson uses data hosted on TU Delft cloud environment. Workshop participants will be given information on how to log-in to the cloud during the workshop.